Pterostilbene inhibits fat accumulation in adipocytes by blocking PPAR-gamma and FAS

  • Pterostilbene inhibits lipid accumulation through cell cycle delay in 3T3-L1 adipocyte
  • Obesity is caused abnormal or excessive fat accumulation that presents a risk to health such as high blood pressure, type 2 diabetes and atherosclerosis. However, recent studies have reported that resveratrol has numerous beneficial effect on cardio-protective, anti-cancer and obesity. Pterostilbene is a stilbenoid and structurally related to resveratrol. Pterostilbene have reported that anti-oxidant, anti-inflammatory, and anti-carcinogenic properties more than resveratrol. Objective: In this study, we examined that pterostilbene inhibits lipid accumulation by cell cycle delay. ABSTRACT: Methods: We investigated Cell viability of pterostilbene through XTT assay. Lipid accumulation of 3T3-L1 checked by using Oil-Red O staining. We experimented western blot to check down regulating obesity-related genes in protein level. Results: Pterostilbene delayed cell cycle in the S phage which is related with delay of cell differentiation. Pterostilbene decreased the accumulation of lipid droplets. The expression of Peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT-enhancer-binding proteins (C/EBPα), and adipocyte protein 2 was decreased by pterostilbene. In addition, pterostilbene reduced lipogenesis (LPAATθ, lipin1, and DGAT1) and fatty acid synthesis (FASN, SREBP) factor in protein level dose dependent manner. Conclusion: Our findings show that pterostilbene inhibits lipid accumulation by regulating adipogenic factors and delaying cell cycle.

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