Hesperidin, leg wellness & cellulite
The flavonoid hesperidin (typically derived from citrus fruits, such as bitter orange) has been widely researched for decades and has been found in numerous studies to help boost circulation and thereby fight water retention. It is also a well-known antioxidant. Hesperidin is therefore of great importance as active ingredient in anti-cellulite, leg wellness and under-eye creams.
HESPERIDIN CELLULITE CREAMS, BY Celluence®
The hesperidin used for the Celluence® cellulite creams is of the highest quality and of >95% purity, i.e. it comprises more than 95% active molecule, in a stabilised, fast-absorbing form. We are extremely proud to feature high quality, highly purified hesperidin in our formulations, together with multiple other natural active ingredients, for maximum synergy and effectiveness. No other cellulite creams offer ALL the important anti-cellulite / leg wellness ingredients, in one package (learn how our creams differ from any other cellulite formulation.
Hesperidin: papers & articles
(Disclaimer: please note that the information and the research presented on this page is for informational purposes only and does not constitute efficacy claims for the Celluence® creams, neither does it constitute or aim to replace medical advice)
- It is well known for several decades now that rheumatoid arthritis is a risk factor for heart disease, both being inflammatory diseases
- In a new study published this week, scientists examined if the combination of hesperidin, a citrus flavonoid, and daidzein, a soya isoflavone, can inhibit markers of both arthritis and heart disease
- Administration of the two phytochemicals for 21 days resulted in reducing multiple markers of inflammation, free radical damage and atherosclerosis / heart disease:
- elastase, the enzyme that breaks elastin
- the inflammatory protein TNF-alpha
- the free radical damage markers malondialdehyde, DPPH and ABTS
- LDL and VLDL cholesterol
- On the other hand, hesperidin and daidzein increased the beneficial HDL cholesterol and total antioxidant levels
- Inclosing the study authors state that the consumption of two compounds, especially hesperidin, "may be helpful in prevention or at least delaying the onset of these diseases in susceptible individuals"
- Source: Anti-arthritogenic and cardioprotective action of hesperidin and daidzein in collagen-induced rheumatoid arthritis
- Abstract: Atherosclerosis has been linked to chronic inflammatory processes. Changes in the levels of lipoproteins, especially low-density lipoprotein or its variants, as well as inflammatory markers, are risk factors for the atherosclerosis. In the present study, an experimental model of rheumatoid arthritis was developed by administrating collagen suspension intradermally in the tail region of Wistar albino rats. At the same time, a suspension of hesperidin (50 mg/kg body weight) and daidzein (20 mg/kg body weight) was orally administrated. The compounds were given in the morning and evening for 21 days. Levels of inflammatory markers in the homogenate of knee joints of experimental rats as well as plasma lipoproteins were investigated. The administration of hesperidin and daidzein caused significant (p < 0.001) decrease in articular elastase activity, TNF-α, and malondialdehyde levels. Further, arthritis scoring and histological findings supported the anti-inflammatory actions of the test compounds. Interestingly, the test compounds also lowered the plasma low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, and triglyceride but increased the level of high-density lipoprotein cholesterol. The test compounds thus ameliorated the risk factors of atherosclerosis. Furthermore, antioxidant roles of hesperidin as well as daidzein were evident from decrease in free radical load demonstrated as increase in total antioxidant level in plasma of arthritic animals treated with hesperidin and daidzein. In a separate in vitro experiment, enhanced free radical scavenging activity of hesperidin was demonstrated against 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid. The anti-inflammatory, hypolipidemic, and antioxidant actions of the naturally occurring test compounds, particularly hesperidin, seem to be quite effective against rheumatoid arthritis and atherosclerosis. Thus, their consumption may be helpful in prevention or at least delaying the onset of these diseases in susceptible individuals.
- Comment: The venotonic and circulation-enhancing medication Daflon (450m Diosmin + 50g hesperidin) has been effectively used for decades for the relief of poor circulation and vein disease in general. This new paper shows that just 50mg of Pycnogenol, a branded red pine bark extract, has the same effect of vein ulcer healing and circulation improvement / oedema reduction.
- Source: Effect of Pycnogenol on the Healing of Venous Ulcers.
- Abstract: BACKGROUND: Venous ulcers are common complications of chronic venous insufficiency that result in severe physical and mental suffering to patients. The oral administration of diosmin/hesperidin has been used as adjuvant therapy in the treatment of chronic venous insufficiency. The purpose of this study was to evaluate and compare the effect of pycnogenol and diosmin/hesperidin on the healing of venous ulcers. METHODS: This longitudinal, prospective, randomized clinical trial was conducted with 30 adult patients with venous ulcers from a vascular surgery outpatient clinic of a university hospital. The patients were randomly allocated to two groups: group 1 (n=15) was treated with pycnogenol (50 mg orally, three-times daily), and group 2 (n=15) was treated with diosmin/hesperidin (450/50 mg orally, twice daily). They were assessed every 15 days for 90 days. During follow-up visits, photo-documentation was obtained and the ulcer area and circumference of the affected limb were measured. Friedman's test and the Mann-Whitney test were used to compare ulcer areas and circumference of affected limbs between and within groups at the different time points. The level of significance was set at 5% (P<0.05) for all tests. RESULTS: Both the pycnogenol and diosmin/hesperidin treatments had a similar effect on the healing of venous ulcers and led to a significant decrease in the circumference of affected limbs (P<0.0001). CONCLUSION: The results suggest that pycnogenol has an adjuvant effect on the healing of venous ulcers, similar to diosmin/hesperidin.
- Source: Long-term Treatment with Hesperidin Improves Endothelium-dependent Vasodilation in Femoral Artery of Spontaneously Hypertensive Rats: The Involvement of NO-synthase and Kv Channels.
- Abstract: Hesperidin is the most common flavonoid found in citrus fruits and is expected to exert vasodilation action relevant to its health benefits. The present study aimed to explore the effect of hesperidin on the vascular responses in normotensive and hypertensive rats and the involvement of NO-synthase and Kv channels. The 15-week-old Wistar and spontaneously hypertensive rats (SHR) were randomized to orally receive either hesperidin (50 mg/kg/day) or a corresponding volume of the water for 4 weeks. Vascular responses of isolated femoral arteries were studied with myograph in control conditions and during inhibition of NO-synthase with l-NNA and Kv channels with 4-AP. Hesperidin had no effect on blood pressure. Endothelium-dependent vasodilation in Wistar and SHR was significantly improved by the treatment with hesperidin. The contraction responses after l-NNA were increased in all groups of rats to similar extent, but relaxatory responses were significantly attenuated only in SHR. The inhibition of Kv channels significantly reduced endothelium-dependent vasodilatory responses in only in SHR administered with hesperidin. The results of our experiment indicate that hesperidin might improve the endothelium-dependent vasodilation during hypertension, possibly through the enhancement of Kv channels function.
- Source: Phytochemicals in regulating fatty acid β-oxidation: Potential underlying mechanisms and their involvement in obesity and weight loss.
- Abstract: Excessive accumulation of fat as the result of more energy intake and less energy expenditure is known as obesity. Lipids are essential components in the human body and are vital for maintaining homeostasis and physiological as well as cellular metabolism. Fatty acid synthesis and catabolism (by fatty acid oxidation) are normal part of basic fuel metabolism in animals. Fatty acids are degraded in the mitochondria by a biochemical process called β-oxidation in which two-carbon fragments are produced in each cycle. The increase in fatty acid oxidation is positively correlated with body mass index. Although healthy life style, avoiding Western diet, dieting and strenuous exercise are the commonly used methods to lose weight, they are not considered a permanent solution in addition to risk attenuation of in basal metabolic rate (BMR). Pharmacotherapy offers benefits of weight loss by altering the satiety and lowering absorption of fat from the food; however, its side effects may outweigh the benefits of weight loss. Alternatively, dietary phytochemicals and natural health products offer great potential as an efficient weight loss strategy by modulating lipid metabolism and/or increasing BMR and thermogenesis. Specifically, polyphenols such as citrus flavonoids, green tea epigallocatechin gallate, resveratrol, capsaicin and curcumin, have been reported to increase lipolysis and induce fatty acid β-oxidation through modulation of hormone sensitive lipase, acetyl-coA carboxylase, carnitine acyl transferase and peroxisome proliferator-activated receptor gamma coactivator-1. In this review article, we discuss selected phytochemicals in relation to their integrated functionalities and specific mechanisms for weight loss.
- Natural actives, such as horse chestnut extract, flavonoids (such as quercetin, hesperidin and rutin), red vine leaves extract, gotu kola / centella asiatica extract and proanthocyanidin, on their own or in combination with compression garments, can offer valuable help in cases of chronic venous insufficiency, lymphedema and circulation impairment, according to this study.
- These actives have been used orally and topically for decades for these purposes, and can offer valuable help in both skin circulation improvement and cellulite reduction (skin microcirculation impairment is an important factor of cellulite).
- Source: A review of the microcirculation in skin in patients with chronic venous insufficiency: the problem and the evidence available for therapeutic options
- Abstract: Impairment of the cutaneous microcirculation is a major predisposing factor in inflammation and ulceration in patients with chronic venous insufficiency (CVI). Increase of capillary filtration rate predisposes to the formation of edema. Local lymphedema is a complication of CVI, often underdiagnosed. This review is focused on CVI but excludes the complication of ulceration. Treatment of microcirculatory dysfunction can be done by pharmacologic intervention or compression therapy or using a combination of both. This review is focused on drugs that have been evaluated by randomized prospective controlled trials. The following compounds are discussed: horse chestnut seed extracts, flavonoids, red vine leaves extracts, total triterpenic fraction of Centella asiatica (L), prociadins, calcium dobesilate, and pentoxifylline. The microcirculatory effects of compression therapy using bandages or stockings are also reviewed. The major microcirculatory effects that have been shown are the reduction of capillary filtration rate and improvements in levels of transcutaneous partial pressures of oxygen and carbon dioxide (TcPO(2) and TcPCO(2)). Available data suggest that a combination of pharmacologic and compression therapy may have some additive effects.
- In a double blind, cross over clinical trial, it was found that the combination of Ruscus aculeatus / (butcher's broom), hesperidin and ascorbic acid (vitamin C) improved vein function and venous disease in the lower legs (calves), especially after taking the supplement.
- Those three natural actives are well known for their vein / circulation supporting action and they are already used in anti-cellulite creams for this purpose.
- Source: Use of extract of Ruscus aculeatus in venous disease in the lower limbs
- Abstract: The effectiveness and tolerability of a venotropic drug (RAES) composed of an extract of Ruscus aculeatus (16.5 mg), hesperidin (75 mg) and ascorbic acid (50 mg) were evaluated in 40 patients (30 female, 10 male) aged between 28 and 74 years, suffering from chronic phlebopathy of the lower limbs. The cross-over, double-blind trial involved two periods of treatment of 2 months with the drug (2 capsules, 3 times/day) or with placebo, and an interim period of 15 days for wash-out. An overall tendency for improvement occurred that was more distinct during the periods of treatment with the drug. In fact, symptoms and plethysmographic parameters (in particular MVIV 40 and 60) immediately changed significantly in correspondence with the administration of RAES. The biological and clinical tolerability were excellent.
- The combination of ruscus aculeatus, vitamin C (ascorbic acid) and hesperidin has been found to improve capillary structure and chronic venous hypertension, and for these reasons it is ideal for inclusion in anti-cellulite / leg wellness creams.
- Source 1: Use of microcirculatory parameters to evaluate clinical treatments of chronic venous disorder (CVD).
- Abstract 1: OBJECTIVES: To evaluate changes on cutaneous microangiopathy in chronic venous disorder (CVD) after use of Cirkan [venotonic drug containing Ruscus aculeatus (plant extract), hesperidine methylchalcone (flavonoid) and vitamin C], elastic compression stockings (ECS) or no treatment for four weeks. PATIENTS AND METHODS: Fifty-five female patients (85 legs), 25 to 57 years, with at least one limb classified as C2,s or C2,3,s (CEAP classification), were allocated consecutively, according to entrance order, in these three groups. Ten healthy women age-matched were also investigated. Using orthogonal polarization spectral technique (noninvasive method), measurements of functional capillary density (FCD, number of capillaries with flowing red blood cells/mm(2)), capillary morphology (CM, % of abnormal capillaries/mm(2)) and diameters (mum) of dermal papilla (DDP), capillary bulk (DCB) and capillary limb (CD) were obtained on the medial perimalleolar region and later analyzed using CapImage software. RESULTS AND CONCLUSIONS: CVD patients showed significant changes on CD and CM compared to healthy subjects in agreement with our previous findings (J Vasc Surg 43:1037-1044, 2006). On Cirkan-treated patients, after 4 weeks, CD decreased on both limbs and CM improved on the left one, suggesting an amelioration of the chronic venous hypertension. No significant changes could be detected on other patient groups. These results confirm the existence of microcirculatory dysfunction in early stages of CVD, probably due to post-capillary hypertension, and further support the venotonic action of Cirkan.
- Source 2: Clinical and capillaroscopic evaluation in the treatment of chronic venous insufficiency with Ruscus aculeatus, hesperidin methylchalcone and ascorbic acid in venous insufficiency treatment of ambulatory patients.
- Abstract AIM: Clinical and capillaroscopic evaluation of an association of Ruscus aculeatus, hesperidin methylchalcone (HMC) and ascorbic acid in chronic venous insufficiency METHODS: A prospective, multicenter and open clinical study. Chronic venous insufficiency patients were studied using clinical, etiological, anatomical, physiological classification (CEAP) symptom scale. Symptomatology, CEAP scale, and baseline, 2-, 4-, 6- and 8-week skin capillaroscopy were assessed. Treatment consisted of two capsules per day of Ruscus aculeatus 150 mg/HMC 150 mg/ascorbic acid 100 mg during 8 weeks. RESULTS: A total of 124 patients were studied, 109 female (89.28%), with a mean age of 52.5 (33-80+9.8). Initial intense reports were 79% pain, 85% heaviness, 74% cramps, 82% edema, decreasing to 20%, 12%, 8% and 14%, respectively, within two weeks, and symptomatology being absent at the end of treatment. Capillaroscopy changes at treatment completion were: 98% to 20% inter-capillary fluid decrease; 80% to 20% efferent loop thickening; 5% to 2% peri-capillary bed, and 5% to 4% mega-capillaries. CONCLUSION: Severe symptom decrease started from the second week until there were no symptoms at the end of treatment. It is the first time morphologic changes were observed in chronic venous insufficiency through capillaroscopy following a pharmacological intervention. Capillary-level effect was proportional to symptom decrease. Improvement was seen from the second week of treatment.
- Source: Hemorrhoids and varicose veins: a review of treatment options
- Abstract: Hemorrhoids and varicose veins are common conditions seen by general practitioners. Both conditions have several treatment modalities for the physician to choose from. Varicose veins are treated with mechanical compression stockings. There are several over-the-counter topical agents available for hemorrhoids. Conservative therapies for both conditions include diet, lifestyle changes, and hydrotherapy which require a high degree of patient compliance to be effective. When conservative hemorrhoid therapy is ineffective, many physicians may choose other non-surgical modalities: injection sclerotherapy, cryotherapy, manual dilation of the anus, infrared photocoagulation, bipolar diathermy, direct current electrocoagulation, or rubber band ligation. Injection sclerotherapy is the non-surgical treatment for primary varicose veins. Non-surgical modalities require physicians to be specially trained, own specialized equipment, and assume associated risks. If a non-surgical approach fails, the patient is often referred to a surgeon. The costly and uncomfortable nature of treatment options often lead a patient to postpone evaluation until aggressive intervention is necessary. Oral dietary supplementation is an attractive addition to the traditional treatment of hemorrhoids and varicose veins. The loss of vascular integrity is associated with the pathogenesis of both hemorrhoids and varicose veins. Several botanical extracts have been shown to improve microcirculation, capillary flow, and vascular tone, and to strengthen the connective tissue of the perivascular amorphous substrate. Oral supplementation with Aesculus hippocastanum, Ruscus aculeatus, Centella asiatica, Hamamelis virginiana, and bioflavonoids may prevent time-consuming, painful, and expensive complications of varicose veins and hemorrhoids.
- In a study comparing patient satisfaction of different therapies of advanced stage chronic venous disease it was found that of the 780 patients studied, and after six weeks of treatment, 71.3% were satisfied after surgical intervention; 51.4% with a natural vasoactive drug (supplement) consisting of ruscus aculeatus (butcher's broom), HMC (hesperidin) and ascorbic acid (vitamin C); and 43% with compression therapy (pressotherapy / bandaging).
- In the drug/supplement group, "out of 377 patients with active venous ulcers smaller than 200 cm2, 18% have been cured, and 66.6% have improved during 6-week period of observation!
- The researchers concluded that effects exerted on veins, capillaries and lymphatic vessels by the vasoactive supplement/drug explain the positive results observed in this study.
- All three components of the drug, i.e. ruscus, ascorbic acid and hesperidin are well researched and widely established for their natural and safe vein / circulation / lymphatic drainage supporting action, and they are valuable ingredients, taken either orally (supplements) or locally (creams) in the fight against venous insufficiency, water retention, poor circulation and cellulite.
- Given that new, more advanced vasoactive supplements exist today, if those newer, more advanced products were used, most probably an even higher number of customer satisfaction would have been recorded.
- Source: Patients' satisfaction with therapy methods of advanced chronic venous disease.
- Abstract AIM: To assess patients' satisfaction from the therapy of advanced chronic venous disorders (CVD) in everyday clinical practice in Poland, and to compare the efficacy of various venoactive drugs (VADs) in venous ulcers healing process. METHODS: 780 unselected adult patients with active (N=441) or healed (N=339) venous ulcers participated in the non-interventional observational 6-week study. RESULTS: Compression therapy and VADs were utilized by 81.5% and 89.2% of patients respectively. 31.2% of all patients underwent surgical procedures for vein incompetence, 61.3% were satisfied with surgical methods, 43% with compression therapy, and 32.6% with VADs - with highest rate of satisfied patients in the group taking Ruscus aculeatus and HMC and ascorbic acid (51.4%). Of 377 patients with active venous ulcers smaller than 200 cm2, adherent to VADs, 18.0% have been cured, and 66.6% have improved during 6-week period of observation. Multiple logistic regression analysis revealed that the compression therapy [OR=2.74], the size of ulcer ≤ 10 cm2 [OR=2.70] were increasing the change of ulcer healing. No VADs was better than another in the healing process. CONCLUSIONS: 1) Compression therapy and VADs are highly utilized by patients with advanced CVD. 2) Patients are more satisfied with surgical than conservative treatment of advanced CVD. 3) More than half of the patients with the advanced stage CVD taking Ruscus aculeatus and HMC and ascorbic acid is satisfied with the obtained improvement. 4) Ruscus aculeatus and HMC and ascorbic acid is similarly effective as other frequently used VADs in venous ulcer healing. 5. Ruscus aculeatus and HMC and ascorbic acid exerting effects on veins, capillaries and lymphatic vessels may explain the positive results observed in this study.
- The combination of Ruscus aculeatus (butcher's broom), hesperidin methyl chalcone (HMC) and ascorbic acid has been tested before on patients with lower limb chronic venous insufficiency and was found effective in reducing water retention and improving vein tone.
- In this study patients with chronic venous disorders (CVD) were assessed after 12 weeks of taking a nutritional supplement comprising of the three actives mentioned above.
- At the conclusion of the study it was found that "all clinical symptoms significantly improved with treatment, especially for patients with higher body mass index and heavier pathology. Ankle circumference, which reveals the extension of water retention decreased over time, gradually decreased and both the physical and psychological dimensions of quality of life significantly increased over time and improved in all types of patients.
- The researchers concluded that" "A 12-week treatment with ruscus aculeatus, HMC and ascorbic acid showed a significant decrease in clinical symptoms and a significant improvement in the quality of life of patients with chronic venous disorder".
- The results are unsurprising, given the well-studied circulation-enhancing and blood vessel-protecting effects these separate actives have on their own.
- Given that poor circulation is an important aspect of cellulite, this combination is also ideal for the treatment of cellulite, both when taken orally (food supplement) or applied locally (cellulite cream). Other active ingredients, in addition to the above three, would offer enhanced synergy and maximum results.
- Source: Quality of life improvement in Latin American patients suffering from chronic venous disorder using a combination of Ruscus aculeatus and hesperidin methyl chalcone and ascorbic acid (quality study).
- Abstract: AIM: The present study assessed the effect of Ruscus aculeatus, hesperidin methyl-chalcone and ascorbic acid (HMC-AA), in the treatment of chronic venous disorders (CVD) in Latin American patients. METHODS: This study is an observational, single arm, multicentric and prospective trial. Patients suffering from CVD and belonging to C0s-C3 Clinical Etiological Anatomical and Physiopathological (CEAP) classes were included. Patient profiles, risk factors, clinical symptomatology and quality of life (QoL) assessed by SF-12 and CIVIQ questionnaires were evaluated at inclusion and after 12-week treatment. RESULTS: The main factors influencing the previous management of patients were age, gender, body mass index (BMI), familial history, physical activity, exposure to heat, heavy loads lifting, profession and clinical characteristics. All clinical symptoms significantly improved with treatment and, as BMI and CEAP classes increased. Ankle circumferences decreased over time, correlating with BMI and CEAP classes. The physical and psychological dimensions of the SF-12 score significantly increased over time and improved within each CEAP class. The CIVIQ score significantly improved over time, correlating with age and CEAP classes. CONCLUSION: A 12-week treatment with Ruscus aculeatus HMC-AA showed a significant decrease in the clinical symptoms and a significant improvement in the QoL of patients with CVD.
- Ruscus aculeaus (butcher's broom) extract is well known for it's action on circulation improvement and chronic vein insufficiency. The flavonoid hesperidin and Vitamin C are also well established vein toning natural chemicals.
- In a study asessing the effectiveness of the combination of these three venotonic chemicals, it was found that patients with chronic venous insufficiency that took 2 capsules a day for 60 days, experienced "significant decrease and regression of clinical symptoms and a reduction in lower limb circumference measured at the ankle were observed."
- Ruscogenin and neoruscogenin, found in butcher's broom extract, are important actives against poor circulation and, as expected, work better in combination with other circulation-enhancing actives. Local application, in the form of microcirculation-boosting or anti-cellulite creams and food supplements is warranted.
- Source: A Study of the Efficacy and Tolerability of a Preparation Containing Ruscus aculeatus in the Treatment of Chronic Venous Insufficiency of the Lower Limbs
- Abstract: Objective: The objective of the study was to assess the efficacy and tolerability of an extract of Ruscus aculeatus combined with hesperidin methyl chalcone and ascorbic acid (Cyclo 3 Fort®) in the treatment of uncomplicated chronic venous insufficiency. Design and Methods: A randomised, double-blind, placebo-controlled study was conducted in 60 patients with uncomplicated chronic venous insufficiency. The patients received two daily capsules of a Ruscus extract combination formulation or placebo for a period of 60 days. Results: During the period of treatment with the Ruscus extract combination formulation, a significant decrease and regression of clinical symptoms and a reduction in lower limb circumference measured at the ankle were observed. The overall assessment of efficacy in the 30 patients receiving the active drug combination showed an excellent result in 15 patients, good in 13 and moderate in two. Of the 30 patients receiving placebo, the results were excellent in four, good in 17, moderate in eight and insufficient in one. Tolerability was considered excellent in 23 cases, good in five and poor in two. Conclusion: It was concluded that Ruscus extract combined with hesperidin methyl chalcone and ascorbic acid is effective and well tolerated in the treatment of patients with uncomplicated venous-lymphatic insufficiency of the lower limbs.
HESPERETIN AND HESPERIDIN STIMULATE FAT RELEASE AND FAT BREAKDOWN - IDEAL FOR CELLULITE REDUCTION
- Hesperetin (a type of flavonoid found in citrus fruits), has been found to fight fat accumulation of fat cells, as well as the growth of new fat cells, and encourages early cell death of fat cells.
- In the body, stem cells can become preadipocytes ("baby" fat cells) and then mature adipocytes (fully grown fat cells), if they receive adipogenic (fat-growing) stimulation from the body. In the body such stimulation is typically provided by a fat-rich diet and inactivity and hormones such as estrogen (natural female hormones or the contraceptive pill), cortisol (stress) and insulin (high-carbohydrate diet).
- For this study the researchers cultivated stem cells in an adipogenic medium designed to stimulate adipogenic conditions in the body. In such a medium, normally a stem cell will become pre-adipocyte and then adipocyte. However, hesperetin stopped stem cells from differentiating into adipocytes and actually encouraged the release of fat (lipolysis) and the premature death of pre-adipocytes (adipocyte apoptosis or adipolysis).
- Due to it's anti-adipogenic, lipolytic and adipolytic effect, hesperetin, along with it's chemical cousin hesperidin, is ideal as an anti-cellulite cream ingredient.
- (Note: The water-insoluble flavonoid hesperidin (hesperetin 7-rutinoside) becomes hesperetin in the body after digestion)
- Source: Hesperetin Inhibit Adipocyte Differentiation and Enhance Bax- and p21-Mediated Adipolysis in Human Mesenchymal Stem Cell Adipogenesis.
- Abstract: We aimed to explore the antiadipogenic and adipolysis effect of hesperetin in human mesenchymal stem cells (hMSCs)-induced adipogenesis. IC50 value of hesperetin was higher for hMSCs such as 149.2 ± 13.2 μmol for 24 h and 89.4 ± 11.4 μmol in 48 h, whereas in preadipocytes was 87.6 ± 9.5 μmol and 72.4 ± 5.6 μmol in 24 h and 48 h, respectively. Hesperetin treatment (5, 10, and 20 μmol) to adipogenesis-induced hMSCs (Group 1) and preadipocytes (Group 2) resulted in a significantly (p < 0.05) increased lipolysis. The treatment with hesperetin decreased the expression of resistin, adiponectin, aP2, LPL, PPAR-γ, and TNF-α in Groups 1 and 2, whereas a significant increase was observed in Bcl, Bax, and p21 expression in Group 2 compared to untreated preadipocytes. hMSCs cultured in adipogenic medium along with hesperetin significantly inhibited adipocyte differentiation and increased the proapoptotic gene expression levels in preadipocyte. Our result indicates the antiadipogenic and adipolysis effects of hesperetin.